CD BioSciences is committed to providing clients with services for the analysis of the geometric characteristics of developing lateral roots. Our expert technical support will help our clients to analyze the character of the growth field of the basal region of the lateral root and to describe the changes in lateral root morphology from a mechanical point of view, providing useful information for their research on plant root growth.

Introduction

Lateral roots occur continuously in the whole life process of plants to adapt to the changing soil environment. The development of lateral roots can be used as an indicator of the ability of plants to adapt to the soil environment. For example, Arabidopsis thaliana supports its growth and development by forming different levels of lateral roots to make full use of soil resources. Therefore, for mature roots, lateral roots are an indispensable part of root function.

The process of lateral root formation involves a large change in its geometry. Experimental data showed that the cross-sectional shape of Arabidopsis lateral roots changes from basal to the apical region. Lateral roots radiate along their own axis. The radiation is associated with the early onset of oblique cell division and leads to a disruption of the symmetry of the cell pattern. From a mechanical point of view, the formation of lateral roots may be considered to be a buckling of the cell wall of the pericycle cells of the parental root that produces the lateral root. The study of the morphological anatomy and geometric features of developing lateral roots is therefore of great theoretical and practical importance.

Fig.1 Real-time analysis of the lateral root primordia (LRP) development using auxin efflux carrier PIN1::PIN1-GFP reporter. (Marhavý, 2015)

Our Services

We offer our clients high-quality services for the analysis of the geometrical characteristics of developing lateral roots through biophysical techniques. Our services include but are not limited to:

• Measurement of the long and short diameters (DL and DS respectively) of the ellipse-like figure representing the base of particular lateral roots and determination of the asymmetry ratio (DL/DS).
• Determination of the growth rate at selected points on the surface of the lateral roots with the application of the growth tensor (GT).
• Obtain and compare growth rate distribution maps for different shapes of basal lateral roots.
• Analysis of the highly dynamic developmental process of lateral root organogenesis by real-time imaging-based methods and investigation of the role of various genetic, hormonal and environmental factors in regulating lateral root organogenesis.
• Mechanical analysis of developing lateral roots.
  ➢ Estimate the critical value of turgor causing the local loss of mechanical stability.
  ➢ Analyze the early growth of the organ under specific assumptions concerning the mechanical properties of the cell wall.
  ➢ Fitting the shape of virtual lateral roots to the shape of real organs.

Advantages

• Experienced and well-trained researchers
• Advanced biophysical technology platforms
• Reliable and cost-effective

CD BioSciences has developed a variety of biophysical analysis methods to help clients characterize the growth of plant lateral roots. We are constantly working to optimize our technology platform to provide our clients with a wider range of biophysical analysis services. If you are interested in our services, please feel free to contact us for more details.

Reference

1. Marhavý, P.; Benková, E. Real-time analysis of lateral root organogenesis in Arabidopsis. Bio-protocol. 2015, 5(8).

• Detection of Root Phenotype
• Measurement of Root Physiological Characteristics
• Analysis of Root Growth Under Mechanical Stress
• Analysis of Plant Root Exudates
• Modeling of Root Growth
• Modeling of Root-Soil Interactions
• AFM-Based Biophysical Analysis of Root Growth