CD BioSciences is committed to developing techniques for biomolecular interaction analysis to help our customers assess affinity during drug development. We offer microscale thermophoresis (MST) technology to determine binding affinity (K_D), stoichiometry and other aspects of interactions, so as to accelerate the progress of customers in drug development.

Background

MST is an emerging technique for the analysis of biomolecular interactions developed based on the thermophoretic effect. The technique measures the affinity between a ligand and a target molecule by observing changes in the conformational size, charge and solvation state of the target molecule after the ligand has bound to it. Elucidating the kinetic, thermodynamic and chemical principles of affinity correlation is very important for high-throughput screening in the early stage of new drug development.

MST has been widely used as a powerful biophysical method in drug discovery studies to analyze and quantify biomolecular interactions. We use the unique properties of MST to rapidly generate binding data for our clients with high accuracy and informative content, while minimizing time and sample consumption. Moreover, the technology has the characteristics of no fixed sample, low sample consumption and easy operation. These advantages make MST a low-risk technology that can be easily integrated into existing workflows.

Fig.1 MST setup and experiments. (Jerabek-Willemsen, 2014)

Our Measurement Services

We provide our clients with MST measurements to analyze and quantify biomolecular interactions. The services we provide include but are not limited to:

• Measurement of molecular motion along a microscopic thermal gradient.
• Determination of affinity, stoichiometry, thermodynamics, stability, mechanism of action (MOA), aggregation.
• Analysis of multicomponent reactions, including competitive effects between multiple binding agents of a target.
• Analysis of complex formation, assembly order and interferences.
• Measurement of thermodynamic parameters.
• Chemically labeling targets to improve detection sensitivity.

MST Assay Service for Drug Discovery

Combining the expertise of MST with our advanced biophysical analysis platform, we are confident that we can offer our clients a wide range of services at different stages of drug discovery.

• Hit identification
• Fragment-based screening
• Assay development
• Hit validation and characterization
• Hit-to-lead

Advantages of Our Technology Platform

• Economical and highly sensitive measurement techniques.
• Suitable for studying binding between any type of biomolecule, including small molecules, multi-protein complexes, liposomes, DNA, nanoparticles, etc.
• Rapid assay development and affinity determination.
• Advanced data acquisition and analysis software support to ensure accurate data processing.

CD BioSciences has built a superior MST platform to provide binding affinity measurement services to our customers. Our specialist biophysical analysis services will provide you with vital data for your research in the field of drug discovery. If you are interested in our services, please contact us for more details.

Reference

1. Jerabek-Willemsen, M. et al. Microscale thermophoresis: Interaction analysis and beyond. Journal of Molecular Structure. 2014, 1077: 101-113.

• Drug Design Based on X-ray Crystallography
• Thermodynamic Characterization of Biomolecular Interactions
• Target Binding Analysis by HDX-MS
• Protein Stability Evaluation by TSA
• Fragment Screening Based on WAC
• Hit Identification by NMR-based Techniques
• Qualification of Targeting Protein-Protein Interactions
• Measurement of Ligand Binding Kinetics
• Identification of Small-Molecule Aggregation
• Analysis of Free Energy Calculations
• Modeling of Scaffold Hopping Transformations
• Cellular Imaging Service for Drug Discovery
• AFM-Based Biophysical Analysis of Drugs