AFM-Based Biophysical Analysis of Pollen Tubes

AFM-Based Biophysical Analysis of Pollen Tubes

The tips of pollen tubes have strong penetration and hyaluronidase, which can pass through the cell walls of pollen receivers and expand rapidly. Understanding the morphological characteristics and mechanical properties of the pollen tube can help to study its growth and pollination mechanism and provide a theoretical basis for improving plant breeding and production. Atomic force microscopy (AFM) is a high-resolution imaging technique that enables observation and analysis of material surfaces at the nanoscale. In the biophysical analysis of pollen tubes, AFM technology enables high-resolution observation of pollen tube morphology and extraction of relevant mechanical parameters such as hardness, elastic modulus, and adhesion.

Fig.1 AFM images of pollen tubes in Torenia fournieri.Fig.1 AFM images of pollen tubes in Torenia fournieri. (Wu JZ, etc., 2008)

CD BioSciences, as a technology company focusing on biophysics, has an advanced AFM platform and is able to provide a variety of biophysical analysis services for pollen tubes. We have conducted in-depth research in the areas of unusual morphological observation, stiffness determination, and multi-angle growth resolution of pollen tubes. Our technical team has many years of experience and is able to tailor our analytical services to meet the specific needs of our customers at different stages and angles of research.

Service Options for AFM-Based Pollen Tubes Biophysical Analysis

We can provide our clients with a variety of biophysical analysis services for pollen tubes, including but not limited to the following:

  • AFM-based high-resolution imaging of pollen tubes
    We are able to provide high-resolution images of pollen tubes to obtain detailed information on pollen tube morphology and characteristic parameters.
  • Hardness and rigidity analysis of pollen tubes
    We can perform mechanical property tests on pollen tubes, such as hardness, modulus of elasticity, surface adhesion and other related parameters, to provide the necessary support for more in-depth studies.
  • Surface chemomechanical analysis of pollen tubes
    We can study specific chemical reactions and interactions on the surface of pollen tubes, such as molecular specific recognition and interaction forces, by using AFM-based chemomechanical force spectroscopy techniques.
  • Thickness analysis of pollen tube cell walls
    We were able to use AFM-based nanoindentation techniques to directly determine the thickness of pollen tube cell walls and to quantitatively characterize the processes that change them under different environmental effects.
  • Nanopore filtration analysis of pollen tube surface
    We are able to analyze the filtration performance of nanopores on the surface of pollen tubes and study their interaction with other small molecules and biomolecules in different environments, and then explore the mechanism of plant nutrient uptake and utilization.


  • Plant reproductive biology research
    To investigate the growth pattern of pollen tubes, molecular recognition mechanism, cell membrane interaction, etc., in order to explore the reproductive biology of plants.
  • Artificial plant reproduction and breeding
    To optimize artificial pollination and breeding techniques to improve the production efficiency and quality of agricultural crops by studying the biophysical characteristics and growth patterns of pollen tubes.
  • Drug development and toxicology research
    To study the structure and interaction on the surface of pollen tubes to find new drug targets or to assess toxic effects.

If you have a pollen tube-related need, please contact CD BioSciences. We will be happy to provide you with the best service, technical support, and data interpretation for your related research.


  1. Wu JZ, Lin Y, Zhang XL, Pang DW, Zhao J. IAA stimulates pollen tube growth and mediates the modification of its wall composition and structure in Torenia fournieri. J Exp Bot. 2008;59(9):2529-43. doi: 10.1093/jxb/ern119. PMID: 18544613; PMCID: PMC2423660.
For research use only, not intended for any clinical use.
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CD BioSciences is a biotechnology company focused on biophysical services. We are proficient in both chemistry and biophysics, and have a comprehensive biophysical platform containing a wide range of advanced technologies.

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