Evaluation of Biophysical Properties of Bone Tissue

Evaluation of Biophysical Properties of Bone Tissue

Evaluation of Biophysical Properties of Bone Tissue

CD BioSciences has developed a world-class biophysical analysis platform to provide our clients with services to assess the biophysical properties of bone tissue, including the detection of bone tissue composition and the analysis of mechanical properties. Our assessment services can be applied to a wide range of animal models to accelerate our clients' research progress in biomedical areas related to skeletal diseases.


Osteoporosis is a systemic bone disease characterized by low bone mass and destruction of bone microarchitecture, leading to increased bone fragility and susceptibility to fracture. Routine clinical outcomes of bone mineral density (BMD) and biochemical markers are commonly used to diagnose osteoporosis and evaluate treatment outcomes. However, these two indicators do not fully explain the observed bone strength. Therefore, the term bone quality, which takes into account the biophysical and biochemical properties associated with bone strength, was introduced to assess normal and osteoporotic bone tissue.

Bone quality includes the structural and material properties of bone. Structural properties are as important as the material properties of the bone in determining the mechanical properties of the bone in health and disease. Vibrational spectroscopy techniques provide a wealth of unique information on bone material properties and structure. Recently, it has been reported that coupling infrared spectroscopy with AFM microscopy allows the study of bone material properties with a lateral spatial resolution of 20-100 nm. It is promising to obtain novel information on bone composition at the nanoscale.

Fig.1 FTIR imaging of the whole cortex of a thin section from an iliac crest biopsy. (Paschalis, 2017)Fig.1 FTIR imaging of the whole cortex of a thin(∼4 μm) section from an iliac crest biopsy. (Paschalis, 2017)

Our Services

Our services include but are not limited to:

  • Assessment of bone tissue-related parameters.
  • Mineral to matrix ratio
  • Mineral maturity/crystallinity
  • Carbonate content
  • Relative tissue water content/nanoporosity
  • Collagen cross-links
  • Relative proteoglycan content
  • Relative lipid content
  • Acid phosphate content
  • Determination of bone mechanical properties such as stiffness (essential for preventing bone bending under a load of body), strength (maximum stress the bone material can sustain before failure) and toughness (absorption of impact energy without reaching complete failure).
  • Multiscale modeling of mechanical biology of bone tissue.

Our Technology Platform

We have established vibrational spectroscopy techniques such as Fourier transform infrared microspectroscopy (FTIRM) and imaging (FTIRI), as well as Raman spectroscopy to help clients determine simultaneous, quantitative and qualitative information on the main bone tissue components in a spatially resolved manner to assess bone quality. At the same time, our advanced technology platform allows the combination of these techniques with other imaging modalities such as time-lapse micro-CT (thus correlating material and structural properties), scanning acoustic microscopy (correlating with mechanical results) and backscattered electron imaging (obtaining mineral distribution), providing both structural and material results.

Our Technology Platform


  • Prediction of fracture incidence.
  • Study on the effect of therapies on bone material properties.
  • Study on correlation between bone quality indices, nanomechanical, and whole bone mechanical properties.
  • Study on pathophysiologic mechanisms of musculoskeletal diseases.

As a leading provider of biophysical analysis services, CD BioSciences has established a variety of appropriate analysis tools that can be used to determine biophysical and biochemical properties related to bone strength. If you need technical support and services in evaluating normal and osteoporotic bone tissue, please contact us for more details.


  1. Paschalis, E. P.; et al. Vibrational spectroscopic techniques to assess bone quality. Osteoporosis International. 2017, 28(8): 2275-2291.
For research use only, not intended for any clinical use.
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CD BioSciences is a biotechnology company focused on biophysical services. We are proficient in both chemistry and biophysics, and have a comprehensive biophysical platform containing a wide range of advanced technologies.

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